浅谈地票交易二级市场构建问题

明确了构建地票交易二级市场的思路,设定了构建地票交易二级市场应首先明确市场主体、客体、交易场所、交易方式、交易流程及规则等市场要素,并在此基础上分析了地票交易二级市场的开放步骤,最后对市场的监管机制进行了设计,以确保地票交易二级市场的平稳、健康运行。     

校园足球背景下我国校园篮球发展的思考和对策

在校园足球得到空前重视和全力推动的大环境里，作为一个篮球教育者，必须去思考篮球的发展与未来。足球、篮球此时的不同待遇有着复杂的现实因素和社会因素，校园足球的发展也将影响校园篮球的发展。在这样的背景下，在对比中思考校园篮球发展的问题所在，从而提出有效的对策。     

氮素营养对马铃薯产量形成特性及品质的影响

为优化旱作马铃薯氮素施用量,进一步提高马铃薯产量和改善薯块品质,通过盆栽种植方式,研究氮素营养对马铃薯干物质积累量、根冠比、产量及薯块品质的影响。结果表明:施氮量为190 kg/hm~2时,马铃薯各生育时期干物质积累量、根冠比和单株产量最高,根据一元二次拟合方程得出最佳施氮量为206.39 kg/hm~2,此时马铃薯单株产量为388.22 g,与实际最佳施氮量接近;马铃薯薯块淀粉和VC含量均在施氮量为190 kg/hm~2时最高,薯块蛋白质含量随氮素的增加呈递增趋势,而有机酸含量随氮素增加呈先增后降的趋势,在施氮量为350 kg/hm~2时达到最大值。在试验条件下,综合分析马铃薯产量形成特性及各品质指标,马铃薯氮素最适施用量为190~206.39 kg/hm~2。     

贵州省黔东南地区草地贪夜蛾寄生性天敌资源调查初报

草地贪夜蛾Spodoptera frugiperda(J. E. Smith)于2019年5月入侵贵州黔东南地区,严重威胁当地农业生态。黔东南地区丰富的生物资源是挖掘草地贪夜蛾自然天敌的重要基础。为明确黔东南地区草地贪夜蛾天敌的发生情况,2019年5月至7月,采用五点取样法对选取的3个地点开展调查。结果表明,草地贪夜蛾在黔东南地区的主要寄生性天敌有5类,分别为白僵菌属、绿僵菌属、茧蜂科、姬蜂科和黑卵蜂属昆虫,寄生率最高分别为45.50%、57.00%、3.00%、20.00%和12.50%,寄生草地贪夜蛾幼虫期4种天敌综合控制力最高达96.50%。本研究可为草地贪夜蛾天敌资源挖掘与利用提供重要参考。     

Cigarette dust particles induced lung function injury of chronic obstructive pulmonary disease in mice

AIM: To investigate the pathogenesis of chronic obstructive disease (COPD) in mice by using nasal drip of cigarette dust particles (DSP) induced pulmonary function damage model.METHODS: BALB/c mice were randomly divided into control group, LPS 100 mg/L group, DSP 0.75 mL/L group, DSP 1.5 mL/L group and DSP 3 mL/L group for 30 days. The method of nasal drip was used for 30 days to establish the COPD model. Rrs, Ers, Crs, Est, Cst, P-3/8Rn, P-3/8G and P-3/8H were measured for evaluating lung function of the mice in each group by the method of FlexiVent. The effect on the increase of airway resistance induced by methacholine (Mach) was determined using main bronchial rings by Myograph method. The HE, Masson and Resorcinol fuchsin staining of mouse tracheas and lung tissues were conducted. RESULTS: Continuous nasal drip with DSP for 30 days increased Rrs, Ers, Est, P-3/8Rn and decreased Crs, Cst, P-3/8G and P-3/8H in the mice. DSP significantly shifted the dose-effect curve of tracheal contraction induced by Mach to the left, increased the sensitivity of the airway to Mach, and significantly increased the maximal contractile airway effect of Mach. Exposure to DSP caused fibrosis of airway subepithelial, deposition of collagen in the airway basement membrane under the reticular plate, induced reticular plate thickening, pulmonary bronchial lumen serious deformation, and the inflammatory cell infiltration in the lung of mice. Significantly increased alveolar wall muscle fibers and collagen fibers were also observed. CONCLUSION: The lung function and pathomorphological changes of COPD mice induced by 30 days nasal drip of cigarette dust particles were similar to those of human COPD.     

2005～2009年我国城市美化植物研究文献计量分析

以《中国期刊全文数据库》为数据源,运用文献计量学的原理和方法,对2005年—2009年国内城市美化植物研究专题文献进行统计分析,探讨这些文献的文献学特征（年代、期刊源和学科方向分布,研究机构特征,科学基金资助,以及合作度与合作率状况）用以明确城市美化植物科研领域的研究能力及今后的研究重点和方向。     

耐低氮糜子品种的筛选及农艺性状的综合评价

【目的】探明耐低氮糜子品种的评价方法,筛选耐低氮糜子基因型材料及鉴定指标,为耐低氮品种的选育和耐低氮生理机制的研究提供理论依据。【方法】采用大田试验,以来自国内外100份糜子品种为材料,在低氮胁迫（0纯氮）和正常施氮（150 kg·hm^-2纯氮）处理下,连续2年进行株高、茎粗、主茎节数、穗长、草重、单株穗重、单株粒重、千粒重、叶面积9个主要农艺性状和氮含量、氮素吸收共11个指标的测定,采用隶属函数法计算各指标耐低氮胁迫指数,通过主成分分析、回归分析与聚类分析评价各糜子品种的综合耐低氮能力。【结果】供试品种在不同氮水平条件下的株高、茎粗、主茎节数、穗长、草重、单株穗重、单株粒重、千粒重、叶面积、氮含量、氮素吸收均存在显著差异;低氮胁迫下,糜子的生长、生物量积累和氮素吸收受到抑制,各性状指标明显下降,变化范围幅度降低,各农艺指标降低幅度排序依次为叶面积>草重>单株粒重>单株穗重>茎粗>主茎节数>穗长>千粒重>株高,不同糜子品种籽粒的氮含量和氮素吸收均降低,降低幅度为氮素吸收>氮含量;低氮胁迫下,不同糜子品种的株高、茎粗、主茎节数、穗长、草重、单株穗重、单株粒重的变异系数大于正常施氮水平各指标的变异系数;不同氮水平下,不同糜子籽粒氮素吸收的变异系数高于氮含量的变异系数,且低氮胁迫的氮素吸收的变异系数高于正常施氮处理。对11个指标的耐低氮胁迫指数进行主成分分析,选择了5个主成分,累积方差贡献率达75.83%;株高、穗长、草重、单株穗重、单株粒重、单株叶面积、氮吸收量的耐低氮胁迫指数与耐低氮综合评价值（D）的相关性均达极显著水平,其中,单株穗重、单株粒重、氮吸收量的相关性较高,其相关系数分别为0.858、0.812和0.812;根据耐低氮综合评价D值,通过聚类分析将100份糜子品种划分为耐低氮型、中间型和不耐低氮型3种类型。【结论】单株穗重、草重、氮吸收量等指标作为糜子耐低氮能力评价的首选指标;榆糜3号、2058、榆黍1号、雁黍7号4个品种耐低氮能力最强。     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

兴山县优质烟叶产区植烟土壤养分动态状况分析

2010年在兴山全县选取1 995个植烟土壤样品进行分析,并与2000年土壤测定结果进行对比,研究兴山县优质烟叶产区(烟区)植烟土壤养分动态变化情况。结果表明,全区土壤有机质含量有所上升,但呈现两级分化趋势,土壤中全氮和碱解氮含量下降,土壤中全磷处于中偏低状态,全烟区普遍缺硼;兴山县烟区应根据"持续控氮、适度增磷,全面补硼"的原则进行施肥调整。     

石榴果实的糖酸组成及风味特点

采用高效液相色谱法测定4种石榴成熟期果实中糖和酸的组成,并对其味感值和糖酸组分与果实风味指标间的相关性进行分析。结果表明,石榴果实中可溶性糖组分有蔗糖、葡萄糖、果糖和山梨醇,其中葡萄糖和果糖含量最高,分别占总糖含量的43.84%~48.36%和49.18%~50.67%。4个品种中,"玉石籽"的总糖含量最高,"白皮"的最低。石榴果实中主要有机酸是奎宁酸、琥珀酸、草酸、L-苹果酸、莽草酸、柠檬酸和D-苹果酸,其中"玛瑙籽"的总酸含量最高,"青皮"的最低。石榴果实中果糖的味感值最大、其次是葡萄糖;味感值最大的酸是奎宁酸,其次为柠檬酸。相关性分析结果表明,石榴果实的糖酸比与甜味/酸味比值之间呈极显著正相关,各品种间糖酸比(甜味/酸味比值)的差异主要取决于有机酸的含量,与糖含量无关。综合其风味特点,"青皮"和"玉石籽"是甜味较浓的品种,"白皮"和"玛瑙籽"则是酸味较浓的品种。